Structure elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 33C, 33D, and revised structure of serotype 33B

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DOIResolve DOI: http://doi.org/10.1016/j.carres.2013.11.006
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TypeArticle
Journal titleCarbohydrate Research
ISSN0008-6215
Volume383
Pages97104; # of pages: 8
SubjectCapsular polysaccharides; Cross reactivity; Serogroup 33; Serotypes; Streptococcus pneumoniae; Structural assignments; Structure elucidation; Sugar residues; Acetylation; Polysaccharides; bacterial polysaccharide; epitope; galactose; glucose; monosaccharide; n acetylgalactosamine; phosphate; pneumococcal capsular polysaccharide; ribitol; ribitol phosphate; unclassified drug; acetylation; article; bacterial capsule; carbohydrate analysis; controlled study; cross reaction; gas chromatography; heteronuclear single quantum coherence; linkage analysis; mass spectrometry; methylation; nonhuman; nuclear magnetic resonance spectroscopy; priority journal; serotype; Streptococcus pneumoniae; Streptococcus pneumoniae
AbstractWe report herein the previously unknown structures of the pneumococcal capsular polysaccharides serotype 33C and 33D, and a revised structure of serotype 33B. The syntenic pair 33B/33D has nearly identical polysaccharide repeat units with the exception of one sugar residue (→2-α-Glcp in 33B and →2-α-Galp in 33D). Serotype 33C is structurally more similar to 33B/33D than 33A/33F, in that it also possesses a backbone ribitol-phosphate group and a →3-β-GalpNAc residue, both of which are absent in the repeat units of 33A/33F. Serotype 33C is notably different from all other serogroup 33 polysaccharides, as there is no →3-β-Glcp residue and the location of the O-acetylation of the →5-β-Galf residue (O-6) differs from the other serogroup 33 polysaccharides (O-2). This completes the structural assignments of polysaccharides within serogroup 33 and provides a framework for understanding the recognition of epitopes by serogroup 33 typing sera based on observed cross-reactivities reported in the literature. © 2013 Elsevier Ltd. All rights reserved.
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LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21270855
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Record identifier67056423-5d4e-4d94-8359-2c836c7a16ee
Record created2014-02-17
Record modified2016-05-09
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