Mouldy feed, mycotoxins and Shiga toxin - producing Escherichia coli colonization associated with Jejunal Hemorrhage Syndrome in beef cattle

  1. Get@NRC: Mouldy feed, mycotoxins and Shiga toxin - producing Escherichia coli colonization associated with Jejunal Hemorrhage Syndrome in beef cattle (Opens in a new window)
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Journal titleBMC Veterinary Research
Article number24
SubjectAnimalia; Aspergillus fumigatus; Bos; Escherichia coli; Fungi; Fusarium; Fusarium poae; Fusarium sporotrichioides; Penicillium roquefortii; mycotoxin; prebiotic agent; animal; animal disease; animal food; article; Aspergillus; cattle; cattle disease; DNA microarray; Escherichia coli infection; food control; Fusarium; gastrointestinal hemorrhage; genetics; isolation and purification; jejunum disease; microbiology; Penicillium; Shiga toxin producing Escherichia coli; syndrome; Animal Feed; Animals; Aspergillus; Cattle; Cattle Diseases; Escherichia coli Infections; Food Microbiology; Fusarium; Gastrointestinal Hemorrhage; Jejunal Diseases; Mycotoxins; Oligonucleotide Array Sequence Analysis; Penicillium; Prebiotics; Shiga-Toxigenic Escherichia coli; Syndrome
AbstractBackground: Both O157 and non-O157 Shiga toxin - producing Escherichia coli (STECs) cause serious human disease outbreaks through the consumption of contaminated foods. Cattle are considered the main reservoir but it is unclear how STECs affect mature animals. Neonatal calves are the susceptible age class for STEC infections causing severe enteritis. In an earlier study, we determined that mycotoxins and STECs were part of the disease complex for dairy cattle with Jejunal Hemorrhage Syndrome (JHS). For STECs to play a role in the development of JHS, we hypothesized that STEC colonization should also be evident in beef cattle with JHS. Aggressive medical and surgical therapies are effective for JHS, but rely on early recognition of clinical signs for optimal outcomes suggesting that novel approaches must be developed for managing this disease. The main objective of this study was to confirm that mouldy feeds, mycotoxins and STEC colonization were associated with the development of JHS in beef cattle.Results: Beef cattle developed JHS after consuming feed containing several types of mycotoxigenic fungi including Fusarium poae, F. verticillioides, F. sporotrichioides, Penicillium roqueforti and Aspergillus fumigatus. Mixtures of STECs colonized the mucosa in the hemorrhaged tissues of the cattle and no other pathogen was identified. The STECs expressed Stx1 and Stx2, but more significantly, Stxs were also present in the blood collected from the lumen of the hemorrhaged jejunum. Feed extracts containing mycotoxins were toxic to enterocytes and 0.1% of a prebiotic, Celmanax Trademark, removed the cytotoxicity in vitro. The inclusion of a prebiotic in the care program for symptomatic beef calves was associated with 69% recovery.Conclusions: The current study confirmed that STECs and mycotoxins are part of the disease complex for JHS in beef cattle. Mycotoxigenic fungi are only relevant in that they produce the mycotoxins deposited in the feed. A prebiotic, Celmanax Trademark, acted as a mycotoxin binder in vitro and interfered with the progression of disease. © 2011 Baines et al; licensee BioMed Central Ltd.
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AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute (BRI-IRB)
Peer reviewedYes
NPARC number21271356
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Record identifier67506dfa-8925-4184-a736-9ec4f64a7202
Record created2014-03-24
Record modified2016-05-09
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