HPLC separation of glucosinolates from leaves and seeds of Arabidopsis thaliana and their identification using thermospray liquid chramatography/mass spectrometry

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DOIResolve DOI: http://doi.org/10.1093/chromsci/26.11.551
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TypeArticle
Journal titleJournal of Chromatographic Science
ISSN0021-9665
Volume26
Issue11
Pages551556; # of pages: 6
SubjectArabidopsis thaliana; glucosinolates
AbstractLeaf and seed extracts of Arabidopsis thaliana var. Columbia contain a large number of glucosinolates, representing close to 25% of those known to occur in nature. The glucosinolates, in the form of their desulphated analogs, are separated by reversed-phase, high-performance liquid chromatography (HPLC). Seventeen are identified using thermospray liquid chromatography/mass spectrometry (TSP LC/MS). Additional glucosinolates, present in trace amounts, are identified as isothiocyanates by electron impact and chemical ionization gas chromatography/MS (GC/MS). In total, 23 glucosinolates are detected and these include four series of homologs and analogs. Fifteen possess aliphatic side chains, of which six contain ω-methylthioalkyl and six contain ω-methylsulphinylalkyl side chains; eight possess aromatic side chains, of which four constitute an homologous series of benzoic acid esters and three possess 3-indolylmethyl-based structures. Sixteen of the glucosinolates are detected in Arabidopsis thaliana for the first time and three of these, 4-hydroxybutyl glucosinolate, 5-benzoyloxypentyl glucosinolate, and 6-benzoyloxyhexyl glucosinolate, represent novel plant constituents.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
NPARC number21274824
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Record identifier67545c04-10be-4e7f-83c3-0c026f56c60a
Record created2015-04-15
Record modified2016-05-09
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