Poly(vinyl alcohol) Functionalized Poly(-dimethylsiloxane) Solid Surface for Immunoassay

Download
  1. Get@NRC: Poly(vinyl alcohol) Functionalized Poly(-dimethylsiloxane) Solid Surface for Immunoassay (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1021/bc060108p
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBioconjugate Chemistry
Volume18
Issue2
Pages281284; # of pages: 4
SubjectAdsorption; Igg; Proteins
AbstractIn this communication, we describe a simple and robust method for the covalent bonding of poly(vinyl alcohol) (PVA) on a silanized poly(dimethylsiloxane) (PDMS) surface. Nonspecific adsorption of proteins via hydrophobic-hydrophobic interactions of the PVA-coated surface is greatly reduced, and biomolecules can be rapidly anchored on the PVA-coated surface with high loading and uniformity. On the basis of a sandwich immunoassay with the anti-rabbit IgG and IgG pair as a model, the detection limit for IgG is down to 1 pg/mL with linearity up to 11 g/mL, the levels often encountered in biological, forensic, and environmental samples.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number49021
NPARC number3539784
Export citationExport as RIS
Report a correctionReport a correction
Record identifier67e7cb05-7784-4770-b6ab-28da39a913b8
Record created2012-01-20
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)
Date modified: