The influence of pore size on osteoblast phenotype expression in cultures grown on porous titanium

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DOIResolve DOI: http://doi.org/10.1016/j.ijom.2012.02.020
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TypeArticle
Journal titleInternational Journal of Oral and Maxillofacial Surgery
Volume41
Issue9
Pages10971101; # of pages: 5
Subjectcell culture; osteoblast; dental implant; pore size; porous titanium
AbstractThis study investigated the effect of pore size on osteoblastic phenotype development in cultures grown on porous titanium (Ti). Porous Ti discs with three different pore sizes, 312 μm (Ti 312), 130 μm (Ti 130) and 62 μm (Ti 62) were fabricated using a powder metallurgy process. Osteoblastic cells obtained from human alveolar bone were cultured on porous Ti samples for periods of up to 14 days. Cell proliferation was affected by pore size at day 3 (p = 0.0010), day 7 (p = 0.0005) and day 10 (p = 0.0090) in the following way: Ti 62 < Ti 130 < Ti 312. Gene expression of bone markers evaluated at 14 days was affected, RUNX2 (p = 0.0153), ALP (p = 0.0153), BSP (p = 0.0156), COL (p = 0.0156), and OPN (p = 0.0156) by pore size as follows: Ti 312 < Ti 130 < Ti 62. Based on these results, the authors suggest that porous Ti surfaces with pore sizes near 62 μm, compared with those of 312 μm and 130 μm, yield the highest expression of osteoblast phenotype as indicated by the lower cell proliferation rate and higher gene expression of bone markers.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Industrial Materials Institute
Peer reviewedYes
NRC number139280
NPARC number21050377
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Record identifier68aa0d4c-29fa-477a-8c2c-36d93942732d
Record created2012-11-29
Record modified2016-05-09
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