Chemoenzymatic synthesis of biotin-appended analogues of gangliosides GM2, GM1, GD1a and GalNAc-GD1a for solid-phase applications and improved ELISA tests

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DOIResolve DOI: http://doi.org/10.1039/C1OB00009H
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TypeArticle
Journal titleOrganic & Biomolecular Chemistry
Volume9
Issue16
Pages58095815; # of pages: 7
AbstractBiotinylated analogues of gangliosides GM2, GM1, GD1a and GalNAc-GD1a were synthesized in high yields using glycosyltransferases from Campylobacter jejuni. The presence of a biotin moiety in the aglycone part of these mimics allows for attachment of these materials onto various streptavidin-coated surfaces. Analysis of the interaction of biotin-appended GM1 with the B subunit of Escherichia coli heat-labile enterotoxin performed in a modified ELISA procedure shows the potential of this compound to replace the natural GM1 in toxin detection.
Publication date
PublisherRSC Publishing
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number19258804
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Record identifier68ba1e4d-bbdd-4ebe-8767-74681992599c
Record created2012-03-08
Record modified2016-05-09
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