Application of Mass Spectrometry to Rapid Analysis of Bacterial Polysaccharides

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TypeArticle
Journal titleIn Comprehensive Natural Products II: Chemistry and Biology
Series titleModern Methods in Natural Products Chemistry; Volume 9
Pages497511; # of pages: 15
AbstractBacterial polysaccharides are present at the outer surface of the bacteria and constitute the principal antigens in most pathogenic bacteria. Comprehensive structural studies have been carried out on a number of capsular polysaccharides and lipopolysaccharides using chemical degradation techniques and nuclear magnetic resonance spectroscopy. However, these studies require milligram quantities of the purified polysaccharide and are not amenable to microanalysis. Mass spectrometry has proven to be a powerful tool for rapid analysis and structural characterization of bacterial polysaccharides on a microscale, including analysis of bacterial cells directly. Of the many mass spectrometric techniques that have been applied to the analysis of polysaccharides, electrospray ionization–mass spectrometry is probably the most significant one. This chapter discusses the present state of our knowledge concerning the methods of isolation, analysis, and mass spectrometry-based characterization of these important biomolecules.
Publication date
PublisherElsevier Science
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biological Sciences
Peer reviewedYes
NPARC number17400943
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Record identifier68f9b466-41e5-4754-ba54-b7f0e71d644d
Record created2011-03-25
Record modified2016-05-09
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