Hydride generation for headspace solid-phase extraction with CdTe quantum dots immobilized on paper for sensitive visual detection of selenium

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DOIResolve DOI: http://doi.org/10.1021/acs.analchem.5b03128
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TypeArticle
Journal titleAnalytical Chemistry
ISSN0003-2700
Volume88
Issue1
Pages789795; # of pages: 7
SubjectBody fluids; Cadmium telluride; Density functional theory; Hydrides; Nanocrystals; Phase separation; Selenium; Semiconductor quantum dots; X ray diffraction; X ray photoelectron spectroscopy; Conventional methods; Headspace solid-phase extraction; Human urine samples; Hydride generations; Potential interferences; Relative standard deviations; Spectrofluorometers; Extraction
AbstractA low-cost, simple, and highly selective analytical method was developed for sensitive visual detection of selenium in human urine both outdoors and at home, by coupling hydride generation with headspace solid-phase extraction using quantum dots (QDs) immobilized on paper. The visible fluorescence from the CdTe QDs immobilized on paper was quenched by H2Se from hydride generation reaction and headspace solid-phase extraction. The potential mechanism was investigated by using X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) as well as Density Functional Theory (DFT). Potential interferences from coexisting ions, particularly Ag+, Cu2+, and Zn2+, were eliminated. The selectivity was significantly increased because the selenium hydride was effectively separated from sample matrices by hydride generation. Moreover, due to the high sampling efficiency of hydride generation and headspace solid phase extraction, the sensitivity and the limit of detection (LOD) were significantly improved compared to conventional methods. A LOD of 0.1 μg L-1 and a relative standard deviation (RSD, n = 7) of 2.4% at a concentration of 20 μg L-1 were obtained when using a commercial spectrofluorometer as the detector. Furthermore, a visual assay based on the proposed method was developed for the detection of Se, 5 μg L-1 of selenium in urine can be discriminated from the blank solution with the naked eye. The proposed method was validated by analysis of certified reference materials and human urine samples with satisfactory results.
Publication date
PublisherACS Publications
LanguageEnglish
AffiliationNational Research Council Canada; Measurement Science and Standards
Peer reviewedYes
NPARC number21277457
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Record identifier69926ad7-0c9d-481e-b89b-6fef6f19f06b
Record created2016-03-09
Record modified2016-05-09
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