A microspore embryogenesis protocol for Camelina sativa, a multi-use crop

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DOIResolve DOI: http://doi.org/10.1007/s11240-011-9948-0
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Journal titlePlant Cell, Tissue and Organ Culture
Pages495501; # of pages: 7
SubjectCamelina sativa; doubled haploidy; haploids; microspore culture; microspore embryogenesis
AbstractCamelina [Camelina sativa (L.) Crantz], a member of the Brassicaceae family, has a unique oil profile that has potential both for biofuels and as a food crop. It is essential to have a doubled haploidy protocol in order to enhance breeding of this crop for prairie conditions as well as improve the yield and quality characteristics, Microspore-derived embryos have been produced from Camelina sativa. Buds 1 – 3 mm in length were selected for culture. The microspores were isolated and purified in full-strength B5 extraction medium and cultured in NLN medium with 12.5% sucrose and 12.5% polyethylene glycol 4000 (PEG) without glutamine, at a density of 10,000 microspores per mL. Glutamine was added to the cultures 72 h after extraction to give a final concentration of 0.8 g/L. The microspore cultures were maintained at 24°C in the dark. After 28 days embryos were observed and these were regenerated to plants and selfed seed was produced. The highest embryogenic frequency achieved was 38 microspore-derived embryos from 100,000 microspores.
Publication date
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
NoteErratum published in Volume 107, Issue 2, page 371, November 2011. DOI: 10.1007/s11240-011-0025-5
Peer reviewedYes
NRC number50179
NPARC number19366509
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Record identifier6b417a6d-d6fd-475e-97f2-6480c375f7f2
Record created2012-04-02
Record modified2016-05-09
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