A clone coding for Schizophyllum commune β-glucosidase: homology with a yeast β-glucosidase

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TypeArticle
Journal titleBiochemistry International
Volume12
Issue6
Pages905912; # of pages: 8
AbstractThree identical clones coding for a partial sequence of the Schizophyllum commune beta-glucosidase were isolated from a cDNA library in lambda gt11, using polyclonal antibody to the enzyme. The identity was confirmed by comparison of the amino-terminus of a peptide from a protease lys-C digest with the sequence inferred from the cDNA sequence. A comparison of the sequence with that reported for a beta-glucosidase from Candida pelliculosa revealed a region in the latter with 43% identity in amino acid sequence. There was also a similarity in the S. commune beta-glucosidase to an active site sequence proposed for a S. commune endoglucanase, suggesting the possibility of a common catalytic mechanism for these two glucolytic enzymes.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberMORANELLI1986
NPARC number9369927
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Record identifier6b8550b0-5432-495f-8a4d-de71d1b03b7a
Record created2009-07-10
Record modified2016-05-09
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