Nanostructured digital microfluidics for enhanced surface plasmon resonance imaging

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Journal titleBiosensors and Bioelectronics
Pages20532059; # of pages: 7
SubjectDiagnostic applications; Digital microfluidics; DNA biochips; Electromagnetic properties; Enhanced surface; Flow cells; Fluidic interconnects; Fluidic manipulation; Infectious disease; Prognostic indicators; Surface Plasmon Resonance imaging; Drop formation; Wetting; DNA hybridization; electromagnetic field; genetic disorder; nanofabrication; Biosensing Techniques; Equipment Failure Analysis; In Situ Hybridization, Fluorescence; Microfluidic Analytical Techniques; Nanostructures; Signal Processing, Computer-Assisted
AbstractThe advances in genomics and proteomics have unveiled an exhaustive catalogue of biomarkers that can potentially be used as diagnostic and prognostic indicators of genetic and infectious diseases. Current thrust in biosensor development is towards rapid, real-time, label-free and highly sensitive detection of the indicative biomarkers. While surface plasmon resonance imaging (SPRi) biosensors could potentially be the best suited candidate for biomarker-based diagnosis, important milestones need to be reached. Commercially available SPRi instrumentation is currently limited by the flow-cell technology to serial-sample processing and has limited sensitivity for the detection of markers present at low concentration. In this paper, we have implemented an approach to enhance sample handling and increase the sensitivity of the SPRi detection technique. We have developed a digital microfluidic platform with an integrated nanostructured biosensor interface that allows for rapid, ultra-low volume, sensitive, and automated on-chip SPRi detection of DNA hybridization reactions. Through the exploitation of electromagnetic properties of nanofabricated periodic gold nanoposts, SPRi signal was increased by 200% with the estimated limit of detection of 500. pM (90 attomoles). Using the versatile fluidic manipulation provided by the digital microfluidics, rapid and parallel target identification was achieved on multiple array elements within 1. min using 180. nL sample volume. By delivering multiple target analytes in individually addressable low volume droplets, without external pumps and fluidic interconnects, the overall assay time, cost and complexity was reduced. The proposed platform allows extreme versatility in the manipulation of precious low volume samples which makes this technology very suitable for diagnostic applications. © 2010 Elsevier B.V.
Publication date
AffiliationNational Research Council Canada (NRC-CNRC); NRC Industrial Materials Institute
Peer reviewedYes
NPARC number21271989
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Record identifier6b9db9fb-692b-44c6-9a8b-ef449a7f5c1b
Record created2014-05-16
Record modified2016-05-09
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