SDS-PAGE for screening non-aggregating human VH domains

Download
  1. Get@NRC: SDS-PAGE for screening non-aggregating human VH domains (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.ab.2010.04.017
AuthorSearch for: ; Search for:
TypeArticle
Journal titleAnalytical Biochemistry
Volume403
Issue1-2
Pages117119; # of pages: 3
AbstractAntibody heavy chain variable domains (VHs) form a significant class of biologics. With VH display libraries—the primary source of VH binders—unwanted aggregating VHs are coselected, sometimes overwhelmingly, alongside nonaggregating VHs. Thus, methods enabling efficient screening for nonaggregating VHs are highly valuable. Here, we found that on nonreducing sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) gels, nonaggregating VHs migrate faster than expected, giving underestimated molecular weights (MWs), whereas aggregating ones migrate slower, giving overestimated MWs. Our finding can be applied to large-scale screening for nonaggregating VHs and possibly other proteins, in particular in display library settings, by SDS–PAGE.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number17401054
Export citationExport as RIS
Report a correctionReport a correction
Record identifier6c4c546b-ea42-449e-8809-02fe738fdb41
Record created2011-03-25
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)