Nutritional requirements for growth of Methanothrix concilii

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DOIResolve DOI: http://doi.org/10.1139/m88-013
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TypeArticle
Journal titleCanadian Journal of Microbiology
Volume34
Pages7377; # of pages: 5
AbstractMethanothrix concilii, which is a mesophilic, aceticlastic methanogen, required 6.8 mM inorganic nitrogen for maximal growth rate in a mineral salts – vitamins medium containing 73.5 mM acetic acid. Cysteine hydrochloride, sodium sulfide, or glutathione at optimum concentrations of 6.0, 1.0, and 12.0 mM, respectively, served as the sole source of sulphur for growth. Of the 10 vitamins tested, only biotin, thiamine hydrochloride, and p-aminobenzoic acid stimulated growth. The optimum iron and nickel concentrations were 1–5 and 0.1 μM, respectively. Both calcium and magnesium, at an optimum concentration of 250 and 500 μM, respectively, were essential for growth. The optimum concentrations of manganese, cobalt, and zinc, in conjunction with one another, were 2.5, 10.0, and 5.0 μM, respectively.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biological Sciences
Peer reviewedNo
NRC numberPATEL1988
28417
NPARC number9361749
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Record identifier6c87e53a-1d65-4aa9-b126-aee765938cf9
Record created2009-07-10
Record modified2016-05-09
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