An introduction to DNA microarrays for gene expression analysis

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DOIResolve DOI: http://doi.org/10.1016/j.chemolab.2010.04.003
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TypeArticle
Journal titleChemometrics and Intelligent Laboratory Systems
Volume104
Issue1
Pages2852; # of pages: 25
SubjectDNA microarray; GeneChip; Gene expression; Experimental design
AbstractThis tutorial presents a basic introduction to DNA microarrays as employed for gene expression analysis, approaching the subject from a chemometrics perspective. The emphasis is on describing the nature of the measurement process, from the platforms used to a few of the standard higher-level data analysis tools employed. Topics include experimental design, detection, image processing, measurement errors, ratio calculation, background correction, normalization, and higher-level data processing. The objective is to present the chemometrician with as clear a picture as possible of an evolving technology so that the strengths and limitations of DNA microarrays are appreciated. Although the focus is primarily on spotted, two-color microarrays, a significant discussion of single-channel, lithographic arrays is also included.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Marine Biosciences
Peer reviewedYes
NRC number51755
NPARC number21268165
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Record identifier6d46e780-f45e-4d81-aac6-4e145e4421c3
Record created2013-05-15
Record modified2016-05-09
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