A role for a complex between activated G protein-coupled receptors in yeast cellular mating

Download
  1. (PDF, 397 KB)
  2. Get@NRC: A role for a complex between activated G protein-coupled receptors in yeast cellular mating (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1073/pnas.0608219104
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue13
Pages53955400; # of pages: 6
SubjectSte2p; Ste3p; protein-protein interaction; membrane juxtaposition
AbstractCell-cell fusion is a fundamental process that facilitates a wide variety of biological events in organisms ranging from yeast to humans. However, relatively little is actually understood with respect to fusion mechanisms. In the model organism Saccharomyces cerevisiae, mating of opposite-type cells is triggered by pheromone activation of the G protein-coupled receptors, α-factor receptor (Ste2p) and a-factor receptor (Ste3p), leading to mitogenactivated protein kinase signaling, growth arrest, and cellular fusion events. Herein we now provide evidence of a role for these receptors in the later cell fusion stage of mating. In vitro assays demonstrated the ability of the receptors to promote mixing of proteoliposomes containing phosphatidylserine, potentially based on a pheromone-dependent interaction between Ste2p and Ste3p that was confirmed by tandem affinity purification and cellular pull-down assays. The cellular mating activity of Ste2p was subsequently probed in vivo. Notably, a receptor-null yeast strain expressing N-terminally truncated Ste2p yielded a phenotype demonstrating wild-type signaling but arrested mating. The arrested prezygotes showed evidence of some cell wall erosion but no membrane juxtaposition at the fusion site. Further, in vitro analyses correlated this mutation with loss of the interaction between Ste2p and Ste3p and inhibition of related lipid mixing. Overall, these results support a role for a complex between activated yeast pheromone receptors in later cell fusion stages of mating, possibly mediating events at the level of cell wall digestion and membrane juxtaposition before membrane fusion.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Plant Biotechnology Institute
Peer reviewedNo
NRC number54670
NPARC number21187429
Export citationExport as RIS
Report a correctionReport a correction
Record identifier7025c24d-34b6-4e76-a087-957c0b8e43c9
Record created2013-01-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)