Nutrient requirement for the degradation of cellulose to methane by a mixed population of anaerobes

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DOIResolve DOI: http://doi.org/10.1099/00221287-112-2-365
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TypeArticle
Journal titleJournal Of General Microbiology
Volume112
Pages365372; # of pages: 8
AbstractSUMMARY: A synthetic medium containing inorganic sources of N, P, Fe, S, Ca2+ and Mg2+, trace minerals and essential vitamins was developed for anaerobic degradation of cellulose to CH4. A mixed culture obtained from sewage sludge and grown in batch culture in this medium degraded about 4 g cellulose l-1 week-1 and produced about 710 ml total gas per g cellulose degraded. The gas produced contained between 51 and 56% CH4, the remainder being CO2. For maximum degradation of cellulose to CH4 and CO2, the requirement for essential salts other than HCO3-, Fe and S2- was comparable to that of other anaerobes. NH4+ or Na2CO3 at 20 to 24 mM and total Fe at 0·4 to 0·6 mM gave optimum conversion of cellulose to CH4, while the inclusion of additional S2- in the presence of 1·75 mM-SO42- was not required.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberKHAN1979
NPARC number9360846
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Record identifier705157aa-f828-4c74-a264-486db0aee904
Record created2009-07-10
Record modified2016-05-09
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