Fluorescence imaging to quantify the fluorescent microspheres in cardiac tissue

Download
  1. (PDF, 1 MB)
  2. Get@NRC: Fluorescence imaging to quantify the fluorescent microspheres in cardiac tissue (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1002/jbio.201000057
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleJournal of Biophotonics
Volume4
Issue4
Pages277287; # of pages: 11
Subjectfluorescence imaging; cardiac tissue; fluorescent microspheres; heart slices
AbstractTo quantify the fluorescent microsphere (FM) content in cardiac tissue, which is an indicative of blood flow, fluorescence imaging of both sides of the pig heart slice was employed. Despite the light scattering inside the tissue and contributions from multiple tissue layers to the total emission, it is shown that the fluorescence intensity at any pixel is proportional to the FM content and the fluorescence image may be transformed to the image of the FM concentration. A convenient standard for the emission-FM concentration transformation is proposed. The approach has several advantages in comparison with the traditional “digestion & extraction” method such as: non-destructiveness, high spatial resolution, high throughput, repeatability and simplicity of operation.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NPARC number19727290
Export citationExport as RIS
Report a correctionReport a correction
Record identifier70638371-7194-44ce-96b5-67675e71c42b
Record created2012-03-28
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)