Scalable production of influenza virus in HEK-293 cells for efficient vaccine manufacturing

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DOIResolve DOI: http://doi.org/10.1016/j.vaccine.2010.03.029
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TypeArticle
Journal titleVaccine
Volume28
Issue21
Pages36613671; # of pages: 11
SubjectAcids; activity; bio; Adenovirus; analysis; Animal; Apoptosis; Bioreactor; Cell Culture; Cell Death; Cell Line; Cells; Centrifugation; Chromatography; Comparative Study; Gene Expression; Gene Therapy; Genes; Glycosylation; HEK-293; Hek-293 cells; Hemagglutinin; Human; I; In Vitro; Influenza; Influenza vaccine; Influenza virus; insect cell; Iodixanol; Kidney; Kinetics; metabolism; methods; Morphogenesis; Neuraminidase; Nucleic Acids; Oxygen; Protein; protein production; Proteins; Purification; Recombinant protein; Recombinant Proteins; Rna; Scalable processes; serum-free medium; Suspension culture; synthesis; therapy; Transfection; Vaccine production; virology; Virulence; Virus production; Virus Replication; Vitro
AbstractCell culture processes offer an attractive alternative to conventional chicken egg-based influenza vaccine production methods. However, most protocols still rely on the use of adherent cells, which makes process scale-up a challenging issue. In this study, it is demonstrated that the HEK-293 human cell line is able to efficiently replicate influenza virus. Production in serum-free suspension of HEK-293 cultures resulted in high titers of infectious influenza viruses for different subtypes and variants including A/H1, A/H3 and B strains. After virus adaptation and optimization of infection conditions, production in 3-L bioreactor resulted in titers of up to 10<sup>9</sup> IVP/mL demonstrating the scale-up potential of the process. Crown Copyright -¬ 2010
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute
Peer reviewedYes
NRC number52752
NPARC number16225341
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Record identifier708c5796-3c2c-4ca2-ae94-4437c91b1829
Record created2010-11-05
Record modified2016-05-09
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