Large-scale production of functional human serum albumin from transgenic rice seeds

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DOIResolve DOI: http://doi.org/10.1073/pnas.1109736108
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TypeArticle
Journal titlePNAS
Volume108
Issue47
Pages1907819083; # of pages: 6
Subjectcrystal structure; recombinant human serum albumin; recombinant protein expression; rice endosperm; recombinant protein processing
AbstractHuman serum albumin (HSA) is widely used in clinical and cell culture applications. Conventional production of HSA from human blood is limited by the availability of blood donation and the high risk of viral transmission from donors. Here, we report the production of Oryza sativa recombinant HSA (OsrHSA) from transgenic rice seeds. The level of OsrHSA reached 10.58% of the total soluble protein of the rice grain. Large-scale production of OsrHSA generated protein with a purity >99% and a productivity rate of 2.75 g/kg brown rice. Physical and biochemical characterization of OsrHSA revealed it to be equivalent to plasma-derived HSA (pHSA). The efficiency of OsrHSA in promoting cell growth and treating liver cirrhosis in rats was similar to that of pHSA. Furthermore, OsrHSA displays similar in vitro and in vivo immunogenicity as pHSA. Our results suggest that a rice seed bioreactor produces cost-effective recombinant HSA that is safe and can help to satisfy an increasing worldwide demand for human serum albumin.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number19254210
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Record identifier70e6ab5d-c360-4e01-8c37-50cf396c1316
Record created2012-03-08
Record modified2016-05-09
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