Sequence determination for N-linked oligosaccharides through the use of the nuclear Overhauser effect: J.Biol.Chem.

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TypeArticle
Journal titleJ.Biol.Chem.
Volume258
Issue3
Pages14311434; # of pages: 4
SubjectALPHA; carbohydrate; Carbohydrate Conformation; Carbohydrate Sequence; CHAIN; CHAINS; chemical; CHEMICAL METHODS; ELUCIDATION; GlcNAc; glycopeptide; Glycopeptides; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; method; Methods; N-linked; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; oligosaccharide; Oligosaccharides; OVERHAUSER; RESIDUES; RESONANCE; SEQUENCE; SEQUENCES; Support,Non-U.S.Gov't; technique
AbstractA nuclear magnetic resonance technique, the nuclear Overhauser effect, has been used to confirm the presence of Man alpha 1-2, GlcNAc beta 1-2, and Man alpha 1-3 residues and to establish the branching pattern and sequences in four classes of N-linked oligosaccharides. This method offers a rapid and nondestructive approach to the elucidation of sequences in carbohydrate chains, in contrast to enzymatic and chemical methods
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberBRISSON1983H
NPARC number9366983
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Record identifier725aa6f4-dc22-4c9e-99ea-3e63fe5429e4
Record created2009-07-10
Record modified2016-05-09
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