Identification and characterization of interactions between abscisic acid and human heat shock protein 70 family members

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DOIResolve DOI: http://doi.org/10.1093/jb/mvt067
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TypeArticle
Journal titleJournal of Biochemistry
ISSN0021-924X
Volume154
Issue4
Pages383391; # of pages: 9
Subjectabscisic acid; chemical proteomics; glucose-regulated protein 78; Heat shock protein 70; protein-ligand interaction
AbstractAbscisic acid (ABA) is a stress-inducible plant hormone comprising an inevitable component of the human diet. Recently, stress-induced accumulation of autocrine ABA was shown in humans, as well as ABA-mediated modulation of a number of disease-associated systems. Now, the application of a chemical proteomics approach to gain further insight into ABA mechanisms of action in mammalian cells is reported. An ABA mimetic photoaffinity probe was applied to intact mammalian insulinoma and embryonic cells, leading to the identification of heat shock protein 70 (HSP70) family members, (including GRP78 and HSP70-2) as putative human ABA-binding proteins. In vitro characterization of the ABA–HSP70 interactions yielded Kds in the 20–60 µM range, which decreased several fold in the presence of co-chaperone. However, ABA was found to have only variable- and co-chaperone-independent effects on the ATPase activity of these proteins. The potential implications of these ABA–HSP70 interactions are discussed with respect to the intracellular protein folding and extracellular receptor-like activities of these stress-inducible proteins. While mechanistic and functional relevance remain enigmatic, we conclude that ABA can bind to human HSP70 family members with physiologically relevant affinities and in a co-chaperone-dependent manner.
Publication date
PublisherOxford University Press
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedYes
NRC number55821
NPARC number21268656
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Record identifier72f3b979-d37f-481f-bf4f-45feaef35002
Record created2013-11-06
Record modified2016-05-09
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