Multimodal nonlinear optical imaging of atherosclerotic plaque development in myocardial infarction prone rabbits

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DOIResolve DOI: http://doi.org/10.1117/1.3353960
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TypeArticle
Journal titleJournal of Biomedical Optics
Volume15
Issue2
Pages020501-1020501-3; # of pages: 3
Subjectatherosclerosis; nonlinear optical microscopy; photonic crystal fiber; coherent anti-Stokes Raman scattering; two-photon excited fluorescence; second harmonic generation
AbstractLabel-free imaging of bulk arterial tissue is demonstrated using a multimodal nonlinear optical microscope based on a photonic crystal fiber and a single femtosecond oscillator operating at 800 nm. Colocalized imaging of extracellular elastin fibers, fibrillar collagen, and lipid-rich structures within aortic tissue obtained from atherosclerosis-prone myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits is demonstrated through two-photon excited fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering, respectively. These images are shown to differentiate healthy arterial wall, early atherosclerotic lesions, and advanced plaques. Clear pathological changes are observed in the extracellular matrix of the arterial wall and correlated with progression of atherosclerotic disease as represented by the age of the WHHLMI rabbits.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biodiagnostics; NRC Steacie Institute for Molecular Sciences
Peer reviewedYes
NRC number20501
NPARC number16488516
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Record identifier73ee8f53-88f4-44ed-a5cc-35e09b6947ac
Record created2011-02-15
Record modified2016-05-09
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