Rapid and simple preparation of a reagentless glucose electrochemical biosensor

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DOIResolve DOI: http://doi.org/10.1039/c2an35128e
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TypeArticle
Journal titleAnalyst
ISSN0003-2654
Volume137
Issue16
Pages38003805; # of pages: 6
Subjectcarbon; glass; glucose; glucose oxidase; immobilized enzyme; article; Aspergillus niger; chemistry; electrochemistry; electrode; enzymology; genetic procedures; metabolism; methodology; time; Aspergillus niger; Biosensing Techniques; Carbon; Electrochemistry; Electrodes; Enzymes, Immobilized; Glass; Glucose; Glucose Oxidase; Time Factors
AbstractA rapid and simple procedure was developed for the preparation of a highly stable and leach-proof glucose oxidase (GOx)-bound glassy carbon electrode (GCE). Crosslinked GOx via glutaraldehyde was drop-cast on a KOH-pretreated GCE followed by drop-casting of 3-aminopropyltriethoxysilane (APTES) to form a stable bioactive layer. At -0.45 V, the biosensor exhibited a wide dynamic detection range of 0.5-48 mM for commercial glucose and 1.3-28.2 mM for Sugar-Chex blood glucose linearity standards. Several endogenous electroactive substances and drug metabolites commonly found in blood were tested and provoked no signal response. To our knowledge, the developed procedure is the most rapid method for preparing a glucose biosensor. The biosensor suffered no biofouling after 7 days of immersion in Sugar-Chex blood glucose. With excellent production reproducibility, GOx-bound electrodes stored dry at room temperature retained their initial activity after several weeks. © 2012 The Royal Society of Chemistry.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute (BRI-IRB)
Peer reviewedYes
NPARC number21269213
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Record identifier749bbafd-4f02-4b1d-924d-68487e96c7f1
Record created2013-12-12
Record modified2016-05-09
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