Light-harvesting system of the red alga Gracilaria tikvahiae. II. Phycobilisome Characteristics of Pigment Mutants

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DOIResolve DOI: http://doi.org/10.1104/pp.73.2.361
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TypeArticle
Journal titlePlant Physiology
ISSN0032-0889
Volume73
Issue2
Pages361369; # of pages: 9
AbstractPhycobilisomes were isolated from wild type Gracilaria tikvahiae and a number of its genetically characterized Mendelian and non-Mendelian pigment mutants in which the principal lesions result in an increase or decrease in the accumulation of phycoerythrin. Both the size and phycoerythrin content of the phycobilisomes are proportional to the phycoerythrin content of the crude algal extracts. In most of the strains examined, the structure and function of the phycocyanin-allophycocyanin phycobilisome cores are the same as in wild type. The phycobilisome architecture is derived from wild type by the addition or removal of phycoerythrin. The same pattern is observed for the phycobilisome of mos2 which contains a large excess of phycocyanin that is not bound to the phycobilisome. The single exception is a yellow, non-Mendelian mutant, NMY-1, which makes functional phycobilisomes composed of phycoerythrin and allophycocyanin with almost no phycocyanin. Characterization of the `linker' polypeptides of the phycobilisome indicates that a 29 kilodalton protein is required for the stable incorporation of phycocyanin into the phycobilisome. Evidence is provided for the requirement of nuclear and cytoplasmic genes in phycobilisome synthesis and assembly. The symmetry properties of the phycobilisome are considered and a structural model for the reaction center II-phycobilisome organization is presented.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
NRC number22524
NPARC number21275096
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Record identifier74ee5153-be21-4a96-9cab-60a0b6cf4893
Record created2015-05-07
Record modified2016-05-09
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