Two-stage cell shrinkage and the OER for radiation-induced apoptosis of rat thymocytes

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DOIResolve DOI: http://doi.org/10.1080/09553009314551791
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TypeArticle
Journal titleInternational Journal of Radiation Biology
ISSN0955-3002
Volume64
IssueNovember 5
Pages571581; # of pages: 11
AbstractSuspensions of rat thymocytes were given 0·09—100 Gy using 60Co γ-rays. The radiation-induced changes in the thymocytes were examined from minutes to hours postirradiation using electron microscopy, agarose gel electrophoresis, staining and Coulter Counter sizing. Sizing by Coulter Counter showed, for the first time, that thymocytes which undergo apoptosis shrink in two distinct stages, first by a sudden decrease from an original volume of 99 µm3 to a volume of 76 µm3, followed by a gradual decrease to 57 µm3 over the space of a few hours. The oxygen enhancement ratio for apoptosis was measured to be about 3·5, similar to the value for reproductive death for many mammalian cells.
Publication date
LanguageEnglish
AffiliationNRC Institute for National Measurement Standards; National Research Council Canada; NRC Institute for Biological Sciences
Peer reviewedNo
Identifier10449681
NRC number482
KLASSEN1993
NPARC number8900062
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Record identifier7538b5e1-90e9-4e85-8791-195683895b77
Record created2009-04-22
Record modified2016-05-09
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