DNA fragmentation in developing lung fibroblasts exposed to Stachybotrys chartarum (atra) toxins

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DOIResolve DOI: http://doi.org/10.1002/ppul.20608
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TypeArticle
Journal titlePediatric Pulmonology
ISSN1099-0496
Volume42
Issue7
Pages592599; # of pages: 8
SubjectStachybotrys chartarum (atra); DNA fragmentation; fetal lung; fibroblasts
AbstractStachybotrys chartarum (atra) is a toxic mold that grows on water-damaged cellulose-based materials. Research has revealed also that inhalation of S. chartarum spores caused marked changes in respiratory epithelium, especially to developing lungs. We analyzed the epigenetic potential of S. chartarum spore toxins on developing rat lung fibroblasts using single cell gel electrophoresis (comet assay). Isolated fetal lung fibroblasts were exposed to S. chartarum spore toxins for 15 min, 3, 14, or 24 hr and control cells were exposed to saline under the same conditions. Cells were embedded in agarose, electrophoresed under alkaline conditions and silver stained. DNA damage was assessed in terms of fragmentation as measured by comet tail length (DNA migration) and intensity (% DNA contained within head and tail). Upon visual inspection, control fibroblasts showed no DNA fragmentation whereas S. chartarum-treated cells had definable comets of various degrees depending upon the time-course. Analyses of the comets revealed that exposure to S. chartarum spore toxins for at least 15 min to 14 hr, induced increased DNA fragmentation in a time-dependent manner. The fact that exposure to toxins for 24 hr showed less damage suggested that developing lung fibroblasts may have the capability of repairing DNA fragmentation. Pediatr Pulmonol. 2007; 42:592–599.
Publication date
PublisherJohn Wiley & Sons, Inc.
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biodiagnostics
Peer reviewedYes
NRC number2348
NPARC number9742571
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Record identifier75c717b4-307c-4afa-be0f-b2668ef91fc1
Record created2009-07-17
Record modified2016-08-12
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