Structural determination and immunochemical characterization of the type V group B Streptococcus capsular polysaccharide: J.Biol.Chem.

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TypeArticle
Journal titleJournal Of Biological Chemistry
Volume266
Issue11
Pages67146719; # of pages: 6
Subject13C; 13C-NMR; 1H; 1H-NMR; ACID; analysis; Antibodies; antibody; bacterial; binding; capsular polysaccharide; capsular polysaccharides; Carbohydrate Conformation; Carbohydrate Sequence; chemistry; complex; COMPLEXES; D-GLUCOSE; epitope; Form; FORMS; group B Streptococcus; GROUP-B; IMMUNOCHEMICAL CHARACTERIZATION; immunology; Indicators and Reagents; isolation & purification; Laboratories; Magnetic Resonance Spectroscopy; Methylation; Methylation analysis; Models,Molecular; Molar; Molecular Sequence Data; NMR; NMR analysis; POLYSACCHARIDE; Polysaccharides; Polysaccharides,Bacterial; REPEATING UNIT; RESIDUES; SIALIC; SIALIC-ACID; Streptococcus; Streptococcus agalactiae; STRUCTURAL; structure; sugar; Support,Non-U.S.Gov't; Support,U.S.Gov't,P.H.S.; UNIT
AbstractThe type V capsular polysaccharide of group B Streptococcus has been isolated and purified, and its repeating unit structure determined. The native type V polysaccharide contains D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, and sialic acid in a molar ratio of 3:2:1:1. Methylation analysis and 1H NMR and 13C NMR analysis of the native type V polysaccharide and of its specifically degraded products permitted the determination of the repeating unit structure of the type V polysaccharide: [formula: see text] The type V polysaccharide has certain structural features in common with other group B streptococcal capsular polysaccharides but is antigenically distinct: no immunologic cross-reactivity was observed between type V and types Ia, Ib, II, III, or IV polysaccharides. Studies of antibody binding to the partially degraded forms of the type V polysaccharide indicated that the native epitope is complex, involving most if not all of the sugar residues of the repeating unit
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberWESSELS1991B
NPARC number9362870
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Record identifier761cc4e5-ff7c-44ec-8205-36f26879ae2a
Record created2009-07-10
Record modified2016-05-09
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