Rapid Microwave Assisted Esterification Method for the Analysis of Poly-3-hydroxybutyrate in Alcaligenes latus by Gas Chromatography

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TypeArticle
Journal titleJournal of Chromatography A
Volume1154
Issue1-2
Pages473476; # of pages: 4
Subjectanalysis; env
AbstractIn the present study, we used previous termmicrowavenext term energy instead of conventional heating to transform poly-3-hydroxybutyrate (PHB) into methyl 3-hydroxybutyrate (Me-3HB) in acidified methanol (H2SO4, 10%, v/v) mixture in less than 4 min at 10% previous termmicrowavenext term power. The previous termmicrowavenext term assisted method was then applied to analyze PHB produced by Alcaligenes latus. The PHB content in the biomass determined using previous termmicrowavenext term heating was comparable to the amount found by conventional heating. Moreover, the new esterification method was at least 50 times faster than the conventional method, affording a significant saving of time and energy.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number49035
NPARC number3539443
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Record identifier765ec23b-8565-4e3a-aefa-317f64a95fd0
Record created2009-03-01
Record modified2016-05-09
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