Polar lipids from the marine macroalga Palmaria palmata inhibit lipopolysaccharide-induced nitric oxide production in RAW264.7 macrophage cells

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DOIResolve DOI: http://doi.org/10.1016/j.phytochem.2014.02.004
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TypeArticle
Journal titlePhytochemistry
ISSN0031-9422
Volume101
Pages101108; # of pages: 8
SubjectMurinae; Palmaria palmata; Palmariaceae; Rhodymenia palmata
AbstractThe EtOAc soluble fraction of a MeOH/CHCl3 extract of Palmaria palmata showed strong nitric oxide (NO) inhibitory activity against lipopolysaccharide (LPS)-induced NO production in murine RAW264.7 cells. NO inhibition-guided isolation led to identification of three new polar lipids including a sulfoquinovosyl diacylglycerol (SQDG) (2S)-1-O-eicosapentaenoyl-2-O- myristoyl-3-O-(6-sulfo-α-d-quinovopyranosyl)-glycerol (1) and two phosphatidylglycerols, 1-O-eicosapentaenoyl-2-O-trans-3-hexadecenoyl-3-phospho- (1′-glycerol)-glycerol (3) and 1-O-eicosapentaenoyl-2-O-palmitoyl-3- phospho-(1′-glycerol)-glycerol (4) from the EtOAc fraction. Seven known lipids were also isolated including a SQDG (2), a phospholipid (5) and five galactolipids (6-10). Structures of the isolated lipids were elucidated by spectral analyses. The isolated SQDGs, phosphatidylglycerols and phospholipid possessed strong and dose-dependent NO inhibitory activity compared to N G-methyl-l-arginine acetate salt (l-NMMA), a well-known NO inhibitor used as a positive control. Further study suggested that these polar lipids suppressed NO production through down-regulation of inducible nitric oxide synthase (iNOS).
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Aquatic and Crop Resource Development
Peer reviewedYes
NRC numberNRC-ACRD-55484
NPARC number21272134
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Record identifier781eae11-4712-4f65-a2b5-455ddb66577e
Record created2014-07-23
Record modified2016-05-09
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