Rapid identification of triacylglycerol-estolides in plant and fungal oils

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DOIResolve DOI: http://doi.org/10.1016/j.indcrop.2011.10.026
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TypeArticle
Journal titleIndustrial Crops and Products
Volume37
Issue1
Pages186194; # of pages: 9
Subjectestolide; Lesquerella fendleri; Lesquerella lyrata; ergot (Claviceps purpurea); seed oil; hydroxy fatty acids
AbstractTriacylglycerol-estolides are components of the storage oil of certain plant and fungal species and are generally associated with the presence of fatty acids containing hydroxyl groups. These unusual acyl-glycerols can easily go undetected when oils are analysed by techniques such as gas chromatography of fatty acid methyl esters, or thin layer chromatography. We describe the detection of TAG-estolides in intact seeds of Lesquerella lyrata and whole sclerotia of the ergot fungus Claviceps purpurea using 1H MAS-NMR for non destructive analysis. We also conducted analysis of small amounts of oil by MALDI-TOF MS to clearly show the presence of TAG-estolides and to rapidly characterize their acyl composition. The matrix used in this work was 2,4,6-trihydroxyacetophenone (THAP) made up in sodium chloride-saturated solvent. We were able to confirm the presence of TAG-estolides with no free hydroxyl groups in the fungal oil, and TAG-estolides with free hydroxyl groups in the oil of Lesquerella lyrata. The development of a technique for the rapid identification of TAG-estolides in oil samples will simplify the detection of these novel lipids in plant and fungal species.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
NRC number20193
NPARC number19547514
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Record identifier7851e344-6ee8-4c3a-ad27-de43a7edf577
Record created2012-04-17
Record modified2016-05-09
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