Comparison of the microbial population dynamics and phylogenetic characterization of a CANOXIS reactor and a UASB reactor degrading trichloroethene

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DOIResolve DOI: http://doi.org/10.1111/j.1365-2672.2004.02475.x
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TypeArticle
Journal titleJournal of Applied Microbiology
ISSN1364-5072
Volume98
Issue2
Pages440449; # of pages: 10
SubjectBacterial complexity; biodegradation; popular dynamics; bacteria, aerobic; bacteria, anaerobic; biodegradation, environmental; bioreactors; population dynamics; soil pollutants; trichloroethylene; hydrogen peroxide; methods; electrophoresis; oxygen; bacteria; methane
AbstractAims: To understand the microbial ecology underlying trichloethene (TCE) degradation in a coupled anaerobic/aerobic single stage (CANOXIS) reactor oxygenated with hydrogen peroxide (H₂O₂) and in an upflow anaerobic sludge bed (UASB) reactor. Methods and Results: The molecular study of the microbial population dynamics and a phylogenetic characterization were conducted using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). In both reactors, TCE had a toxic effect on two uncultured bacterial populations whereas oxygen favoured the growth of aerobic species belonging to Rhizobiaceae and Dechloromonas. No methanotrophic bacteria were detected when targeting 16S rRNA gene with universal primers. Alternatively, pmo gene encoding the particulate methane monooxygenase of Methylomonas sp. LW21 could be detected in the coupled reactor when H₂O₂ was supplied at 0·7 g O₂ 1R⁻¹ day⁻¹. Conclusions: Methylomonas sp. LW21 that could be responsible for the aerobic degradation of the TCE by-products is not among the predominant bacterial populations in the coupled reactor. It seems to have been outcompeted by heterotrophic bacteria (Rhizobiaceae and Dechloromonas sp.) for oxygen. Significance and Impact of the Study: The results obtained show the limitations of the coupled reactor examined in this study. Further investigations should focus on the operating conditions of this reactor in order to favour the growth of the methanotrophs.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
Identifier1689134x
NRC number45916
NPARC number12338629
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Record identifier789cca14-673c-4105-a110-073d4c456e97
Record created2009-09-10
Record modified2016-05-09
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