Comparison between various commercial sources of almond beta-glucosidase for the production of alkyl glucosides

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DOIResolve DOI: http://doi.org/10.1016/j.molcatb.2005.11.012
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TypeArticle
Journal titleJournal of Molecular Catalysis B : Enzymatic
Volume38
Issue2
Pages9194; # of pages: 4
AbstractTen different almond β-glucosidases, from eight commercial sources, were compared for their capacity to produce octyl glucoside by direct condensation from glucose and octanol, with and without added dimethyl formamide (DMF) in the octanol media. All the preparations tested catalyzed the synthesis of octyl glucoside, at rates ranging from 0.002 to 0.012 μmol/h, per added unit of enzyme, one unit being defined as the amount of enzyme catalyzing the hydrolysis of 1 μmol of p-nitrophenyl-β-d-glucopyranoside (p-NPG) per minute at 50 °C at pH 4.8. However, none of the preparations showed strong activation by DMF, contrary to what has been previously observed. Some preparations were slightly activated (two-fold maximum) by 10% DMF. It appears that the enzymes with the lowest specific activity for the hydrolysis of p-NPG were activated more strongly by DMF than the most active preparations.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NPARC number12339284
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Record identifier79ebdc39-6efa-4a0f-9e2d-14e15419d5e3
Record created2009-09-11
Record modified2016-05-09
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