Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay

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DOIResolve DOI: http://doi.org/10.1016/j.ab.2011.05.045
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TypeArticle
Journal titleAnalytical Biochemistry
Volume417
Issue1
Pages156158; # of pages: 3
AbstractThis study revealed a major interference from sulfo-N-hydroxysuccinimide (sulfo-NHS) in the bicinchoninic acid (BCA) protein assay. Sulfo-NHS, a common reagent used in bioconjugation and analytical biochemistry, exhibited absorbance signals and absorbance peaks at 562 nm, comparable to bovine serum albumin (BSA). However, the combined absorbance of sulfo-NHS and BSA was not strictly additive. The sulfo-NHS interference was suggested to be caused by the reduction of Cu2+ in the BCA Kit's reagent B (4 percent cupric sulfate) in a manner similar to that of the protein.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number53375
NPARC number18969351
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Record identifier7bdf1f77-dff2-4475-8fb5-f4066a26d71e
Record created2012-03-06
Record modified2016-05-09
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