An extract of the marine alga Alaria esculenta modulates α-synuclein folding and amyloid formation

Download
  1. Get@NRC: An extract of the marine alga Alaria esculenta modulates α-synuclein folding and amyloid formation (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.neulet.2017.02.055
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleNeuroscience Letters
ISSN0304-3940
Volume644
Pages8793
SubjectParkinson’s disease; alpha-synuclein; amyloid; protein misfolding; brown seaweed; Alaria esculenta
AbstractThe conversion of α-synuclein from its natively unfolded and α-helical tetrameric forms to an amyloid conformation is central to the emergence of Parkinson’s disease. Therefore, prevention of this conversion may offer an effective way of avoiding the onset of this disease or delaying its progress. At different concentrations, an aqueous extract from the edible winged kelp (Alaria esculenta), was shown to lower and to raise the melting point of α-synuclein. Size fractionation of the extract resulted in the separation of these distinct activities. The fraction below 5 kDa decreased the melting point of α-synuclein, whereas the fraction above 10 kDa raised the melting point. Both of these fractions were found to inhibit the formation of amyloid aggregates by α-synuclein, measured by thioflavin T dye-binding assays; this effect was further confirmed by transmission electron microscopy showing the inhibition of fibril formation. Circular dichroism analysis suggested that the incubation of α-synuclein under fibrillation conditions resulted in the loss of substantial native helical structure in the presence and absence of the fractions. It is therefore likely that the fractions inhibit fibrillation by interacting with the unfolded form of α-synuclein.
Publication date
PublisherElsevier
LanguageEnglish
AffiliationAquatic and Crop Resource Development; National Research Council Canada
Peer reviewedYes
NRC numberACRD 56233
NPARC number23001761
Export citationExport as RIS
Report a correctionReport a correction
Record identifier7c9fdd39-0790-4892-982f-aa169ed7df75
Record created2017-03-29
Record modified2017-03-29
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)