Enzymatic hydrolysis of nitrides by an engineered nitrile hydratase (Papain Gln19Glu) in aqueous-organic media

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DOIResolve DOI: http://doi.org/10.1002/bit.10267
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TypeArticle
Journal titleBiotechnology and Bioengineering
Volume79
Issue1
Pages914; # of pages: 6
AbstractA mutant of the cysteine protease papain, displaying nitrile hydratase and amidase activities, was expressed in and used for the hydrolysis of peptide nitriles in aqueous-organic media. The rate of hydrolysis of these nitriles is lowered by increasing acetone concentration. This is caused by an increase of the Michaelis constant, and a variation of max proportional to the amount of water in the mixture. The hydrolysis of the amide is less affected by the increase in co-solvent, which results in lower accumulation of this intermediate product. With the peptide nitrile tested, high nitrile concentrations could be used to promote the production of the amide and prevent its hydrolysis to the acid by diminishing the relative rate of amide hydrolysis. A number of non-peptidyl nitriles were also tested as potential substrates but activity was detected for only one compound with structural resemblance to peptide nitriles. � 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 9-14, 2002.
Publication date
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NPARC number12328466
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Record identifier7d56273d-bfbb-4638-a6af-0ed9f9834f78
Record created2009-09-10
Record modified2016-05-09
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