Effects of stem girdling on cone yield and endogenous phytohormones and metabolites in developing long shoots of Douglas-fir (Pseudotsuga menziesii)

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DOIResolve DOI: http://doi.org/10.1007/s11056-011-9294-4
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TypeArticle
Journal titleNew Forests
ISSN0169-4286
Volume43
Issue4
Pages491503; # of pages: 13
Subjectcone yield; Douglas-fir; girdling; genotype; metabolites; phytohormones
AbstractStem-girdling treatments were applied in early spring to stimulate cone formation in two genotypes of interior Douglas-fir (Pseudotsuga menziesii var. glauca (Beissn.) Franco). After girdling treatments, male cone yield increased significantly in the next growing season. The increase was 14-fold in genotype 9137. In genotype 9550, more than 8,700 male cones were induced from each tree whereas no male cones were found in controls. Female cone yield was zero in controls and low for girdled trees in both genotypes. Multiple phytohormone-related compounds, including gibberellins (GAs), cytokinins, indole-3-acetic acid (IAA), abscisic acid (ABA) and their selected metabolites, were analyzed in developing long shoots after girdling treatments by high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) in multiple reaction monitoring mode. Concentrations of GA4 were slightly higher at week 2 following girdling treatment, whereas at week 8 lower GA4 concentrations were found in girdled samples. Stem girdling did not affect concentrations of IAA and major cytokinins, such as zeatin riboside and isopentenyl adenosine. Concentrations of ABA differed two-fold between the genotypes. Although girdling treatment did not cause differences in ABA concentrations, it generally resulted in higher concentrations of ABA glucose ester. Concentration increase of 7'-hydroxy ABA by girdling was only found in genotype 9550 at week 8. Girdling caused little change in concentrations of phaseic acid in both genotypes.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
NRC number54645
NPARC number19727304
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Record identifier7d57d5d1-298c-4c23-96d2-95e1561f065b
Record created2012-04-02
Record modified2016-05-09
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