Antigenic variation among Bordetella : Bordetella bronchiseptica strain MO149 expresses a novel O chain that is poorly immunogenic

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DOIResolve DOI: http://doi.org/10.1074/jbc.M110.115121
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TypeArticle
Journal titleJournal Of Biological Chemistry
Volume285
Issue35
Pages2686926877; # of pages: 9
AbstractThe O chain polysaccharide (O PS) of Bordetella bronchiseptica and Bordetella parapertussis lipopolysaccharide is a homopolymer of 2,3-diacetamido-2,3-dideoxygalacturonic acid (GalNAc3NAcA) in which some of the sugars are present as uronamides. The terminal residue contains several unusual modifications. To date, two types of modification have been characterized, and a survey of numerous strains demonstrated that each contained one of these two modification types. Host antibody responses against the O PS are directed against the terminal residue modifications, and there is little cross-reactivity between the two types. This suggests that Bordetella O PS modifications represent a means of antigenic variation. Here we report the characterization of the O PS of B. bronchiseptica strain MO149. It consists of a novel two-sugar repeating unit and a novel terminal residue modification, with the structure Me-4-α-L-GalNAc3NAcA-(4-β-D-GlcNAc3NAcA-4-α-LGalNAc3NAcA-) 5–6-, which we propose be defined as the B. bronchiseptica O3 PS. We show that the O3 PS is very poorly immunogenic and that the MO149 strain contains a novel wbm (O PS biosynthesis) locus. Thus, there is greater diversity among Bordetella O PSs than previously recognized, which is likely to be a result of selection pressure from host immunity. We also determine experimentally, for the first time, the absolute configuration of the diacetimido-uronic acid sugars in BordetellaOPS.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number17653000
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Record identifier7e0e77a7-e406-49c5-b99a-3e1d6715a79b
Record created2011-04-01
Record modified2016-05-09
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