Structural determination of the O-antigenic polysaccharide of enteropathogenic Escherichia coli O103:H2

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DOIResolve DOI: http://doi.org/10.1139/W10-015
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TypeArticle
Journal titleCanadian Journal of Microbiology
Volume56
Issue5
Pages366372; # of pages: 7
SubjectEscherichia coli O103:H2; lipopolysaccharide; O-antigen; NMR
AbstractThe structure of the antigenic O-polysaccharide isolated from the lipopolysaccharide produced by enterohemorrhagic Escherichia coli O103:H2 was determined and shown to be composed of D-glucose (1 part), 2-acetamido-2-deoxy-Dglucose (2 parts), 2-acetamido-2-deoxy-D-galactose (1 part), and 3-deoxy-3-(R)-3-hydroxybutyramido-D-fucose (1 part). From the results of methylation analysis, Smith-type periodate oxidation degradation studies, and the use of one- and twodimensional 1H and 13C NMR spectroscopy, the O-polysaccharide antigen was found to be an unbranched polymer of a repeating pentasaccharide unit having the following structure: ->2)-b-D-Glcp-(1->2)-b-D-Fucp3NBu-(1->6)-a-D-GlcpNAc- (1->4)-a-D-GalpNAc-(1->3)-b-D-GlcpNAc-(1->, where Bu is (R)-3-hydroxybutyramido.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number17653003
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Record identifier7efca11f-20e4-45a2-bea5-527ece90b200
Record created2011-04-01
Record modified2016-05-10
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