Construction of genetic linkage map and mapping of QTL for seed color in Brassica rapa

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DOIResolve DOI: http://doi.org/10.1139/g2012-066
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TypeArticle
Journal titleGenome
ISSN0831-2796
Volume55
Issue12
Pages813823; # of pages: 11
SubjectBrassica rapa; yellow sarson; seed color; linkage map; QTL mapping; yellow sarson; couleur des graines; carte génétique; cartographie QTL
AbstractA genetic linkage map of Brassica rapa L. was constructed using recombinant inbred lines (RILs) derived from a cross between yellow-seeded cultivar Sampad and a yellowish brown seeded inbred line 3-0026.027. The RILs were evaluated for seed color under three conditions: field plot, greenhouse, and controlled growth chambers. Variation for seed color in the RILs ranged from yellow, like yellow sarson, to dark brown/black even though neither parent had shown brown/black colored seeds. One major QTL (SCA9-2) and one minor QTL (SCA9-1) on linkage group (LG) A9 and two minor QTL (SCA3-1, SCA5-1) on LG A3 and LG A5, respectively, were detected. These collectively explained about 67% of the total phenotypic variance. SCA9-2 mapped in the middle of LG A9, explained about 55% phenotypic variance, and consistently expressed in all environments. The second QTL on LG A9 was ~70 cM away from SCA9-2, suggesting that independent assortment of these QTLs is possible. A digenic epistatic interaction was found between the two main effect QTL on LG A9; and the epistasis X environment interaction was nonsignificant, suggesting stability of the interaction across the environments. The QTL effect on LG A9 was validated using simple sequence repeat (SSR) markers from the two QTL regions of this LG on a B₁S₁ population (F₁ backcrossed to Sampad followed by self-pollination) segregating for brown and yellow seed color, and on their self-pollinated progenies (B₁S₂). The SSR markers from the QTL region SCA9-2 showed a stronger linkage association with seed color as compared with the marker from SCA9-1. This suggests that the QTL SCA9-2 is the major determinant of seed color in the A genome of B. rapa.
Publication date
PublisherNational Research Council Canada. Research Press
LanguageEnglish
AffiliationNational Research Council Canada; NRC Genomics and Health Initiative; Aquatic and Crop Resource Development
Peer reviewedYes
NRC number54671
NPARC number21268489
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Record identifier8157ccbb-7148-44d7-ae09-d3d479f59b74
Record created2013-08-15
Record modified2016-10-04
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