Isolation of mycoparasitic-related transcripts by SSH during interaction of the mycoparasite Stachybotrys elegans with its host Rhizoctonia solani

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DOIResolve DOI: http://doi.org/10.1007/s00294-007-0166-6
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TypeArticle
Journal titleCurrent Genetics
Volume53
Issue2
Pages6780; # of pages: 14
Subjectbio; crossector; env; Biotechnology; physiology; analysis; Genes; pathogenicity; metabolism; genome; Gene Expression; mycoparasitism; SSH; differential screening; gene expression; mycoparasitism-induced genes; ESTs
AbstractMycoparasitism by antagonistic fungi involves changes in the biochemistry and physiology of both partners. Analysis of genes that are expressed during mycoparasite– host interaction represents a powerful strategy to obtain insight into the molecular events underlying these changes. The aim of this study is to identify genes whose expression is upregulated when the mycoparasite Stachybotrys elegans is in direct confrontation with its host Rhizoctonia solani. Suppression subtractive hybridization (SSH) was used to create a subtracted cDNA library, and differential screening was applied to identify the over-expressed transcripts. We report the analysis of 2,166 clones, among which 47% were upregulated during mycoparasitism. Two hundred and sixtyone clones were sequenced that corresponded to 94 unique genes. Forty-four of these were identified as novel genes, while the remainder showed similarity to a broad diversity of genes with putative functions related to toxin production, pathogenicity, and metabolism. As a result of mycoparasitism, 15 genes belonged to R. solani among which 9 genes were assigned putative functions. Quantitative RT-PCR was used to examine the upregulation of 12 genes during the course of mycoparasitism. Seven genes showed significant upregulation at least at one-time point during interaction of the mycoparasite with its host. This study describes a first step toward knowledge of S. elegans genome. The results present the useful application of EST analysis on S. elegans and provide preliminary indication of gene expression putatively involved in mycoparasitism.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute (BRI-IRB); National Research Council Canada
Peer reviewedNo
NRC number47838
NPARC number12400999
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Record identifier81f8fc07-387a-407e-b47d-a088ee20a885
Record created2009-10-26
Record modified2016-05-09
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