Analysis of gene expression profiles of two near-isogenic lines differing at a QTL region affecting oil content at high temperatures during seed maturation in oilseed rape (Brassica napus L.)

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DOIResolve DOI: http://doi.org/10.1007/s00122-011-1725-2
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TypeArticle
Journal titleTheoretical and Applied Genetics
ISSN0040-5752
1432-2242
Volume124
Issue3
Pages515531; # of pages: 17
SubjectBrassica napus; Developing seed; Down-regulation; Gene expression profiles; Genetic divergence; Gibberellic acid; Growth chamber; High temperature; Lipid metabolisms; Microarray analysis; Molecular mechanism; Near isogenic lines; Oil contents; Oil seed rape; Red light; Seed maturation; Seed oil; Transcriptomes; Translational elongation
AbstractSeed oil production in oilseed rape is greatly affected by the temperature during seed maturation. However, the molecular mechanism of the interaction between genotype and temperature in seed maturation remains largely unknown. We developed two near-isogenic lines (NIL-9 and NIL-1), differing mainly at a QTL region influencing oil content on Brassica napus chromosome C2 (qOC.C2.2) under high temperature during seed maturation. The NILs were treated under different temperatures in a growth chamber after flowering. RNA from developing seeds was extracted on the 25th day after flowering (DAF), and transcriptomes were determined by microarray analysis. Statistical analysis indicated that genotype, temperature, and the interaction between genotype and temperature (G × T) all significantly affected the expression of the genes in the 25 DAF seeds, resulting in 4,982, 19,111, and 839 differentially expressed unisequences, respectively. NIL-9 had higher seed oil content than NIL-1 under all of the temperatures in the experiments, especially at high temperatures. A total of 39 genes, among which six are located at qOC.C2.2, were differentially expressed among the NILs regardless of temperature, indicating the core genetic divergence that was unaffected by temperature. Increasing the temperature caused a reduction in seed oil content that was accompanied by the downregulation of a number of genes associated with red light response, photosynthesis, response to gibberellic acid stimulus, and translational elongation, as well as several genes of importance in the lipid metabolism pathway. These results contribute to our knowledge of the molecular nature of QTLs and the interaction between genotype and temperature.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
Identifier1725
NRC number54642
NPARC number21268253
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Record identifier8323e376-7136-4b3e-9e1a-927b08a0cdb6
Record created2013-06-10
Record modified2016-05-09
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