Nonfouling hydrophilic poly(ethylene glycol) engraftment strategy for PDMS/SU-8 heterogeneous microfluidic devices

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DOIResolve DOI: http://doi.org/10.1021/la303196m
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TypeArticle
Journal titleLangmuir
ISSN0743-7463
1520-5827
Volume28
Issue46
Pages1622716236
AbstractWe report a novel nonfouling passivation method using poly(ethylene glycol) (PEG) engraftment on the surfaces of poly(dimethylsiloxane) (PDMS) microfluidic devices sealed with SU-8. To achieve bonding between the PDMS and SU-8 surfaces, the PDMS surface was first functionalized with amines by treatment with 3-aminopropyltrimethoxysilane (APTMS) for subsequent reaction with epoxide functional groups on SU-8 surfaces. To modify the heterogeneous surfaces of the resulting PDMS/SU-8 microfluidic device further, the remaining SU-8 surfaces were amino functionalized using ethylene diamine (EDA), followed by treating both amino-functionalized PDMS and SU-8 surfaces with mPEG-NHS (N-hydroxysuccinimide) through an amine-NHS reaction for facile PEG immobilizations, thus simultaneously modifying both PDMS and SU-8 surfaces in one reaction. Detailed surface analyses such as the water contact angle, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM) were conducted to confirm the chemical reactions and characterize the resulting surface properties. To test the efficacy of this surface-modification strategy, we conducted nonspecific protein and particle binding tests using microfluidic devices with and without modifications. The PEG-modified PDMS/SU-8 device surfaces showed a 64.5% reduction in nonspecific bovine serum albumin (BSA) adsorption in comparison to that of the unmodified surfaces and 92.0 and 95.8% reductions in microbead adhesion under both stagnant and flowing conditions, respectively.
Publication date
LanguageEnglish
AffiliationInformation and Communication Technologies; National Research Council Canada
Peer reviewedNo
NPARC number21268905
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Record identifier842fde97-4d2f-4da5-a1b1-0aca031ae4fc
Record created2013-11-25
Record modified2016-05-09
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