Estimation of the location of natural α-tocopherol in lipid bilayers by 13C-NMR spectroscopy

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DOIResolve DOI: http://doi.org/10.1016/0005-2736(85)90203-2
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TypeArticle
Journal titleBBA - Biomembranes
ISSN0005-2736
Volume819
Issue1
Pages131135; # of pages: 5
Subjectalpha tocopherol; animal cell; chicken; female genital system; lipid membrane; nonhuman; nuclear magnetic resonance; priority journal; Lipid Bilayers; Magnetic Resonance Spectroscopy; Membrane Fluidity; Phosphatidylcholines; Phospholipids; Vitamin E; Animalia; Gallus gallus
AbstractNatural, 2R,4′R,8′R-α-tocopherol (vitamin E), labelled selectively with 13C in the methyl group at position 5, was incorporated into unilamellar vesicles of egg phosphatidylcholine. The vesicles are impermeable to the shift reagent Pr3+ and, in the presence of this reagent, separate 13C resonances due to labelled α-tocopherol in the outer and inner monolayers could be observed with relative intensities, 2:1. Subsequent addition of the relaxation reagent Gd2+ causes broadening and greatly shortened spin-lattice relaxation times for the resonance due to α-tocopherol in the outer monolayer only. These data confirm that α-tocopherol is located in both halves of the bilayers with its more hydrophilic chroman moiety very near the lipid-water interface, and indicate that the methyl group at position 5 of the α-tocopherol in the inner monolayer must be at least 40 Å from the aqueous interface of the outer monolayer. © 1985.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21276756
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Record identifier85f63267-818c-4d68-8d40-fbd6c20d5bbf
Record created2015-10-13
Record modified2016-05-09
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