The structure of the O-antigen in the endotoxin of the emerging food pathogen Cronobacter (Enterobacter) muytjensii strain 3270

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DOIResolve DOI: http://doi.org/10.1016/j.carres.2009.01.020
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TypeArticle
Journal titleCarbohydrate Research
Volume344
Issue5
Pages667671; # of pages: 5
SubjectO-Polysaccharide; cronobacter sakazakii; cronobacter muytjensii; NMR; LPS; o-antigen; 13C; 13C-NMR; 1H; 2D; abscess; acid; bacteria; bacterial; brain; Canada; chemical; disease; endotoxin; gram negative Bacteria; gram-negative bcteria; identification; infant; linear; lipopolysaccharide; lipopolysaccharides; meningitis; method; methods; MS; strain; strains; structural; structure; unit
AbstractStrains of the Gram-negative bacterium Cronobacter (formerly known as Enterobacter) sakazakii have been identified as emerging opportunistic pathogens that can cause enterocolitis, bacteraemia, meningitis, and brain abscess, and they have been particularly associated with meningitis in neonates where infant milk formulae have been epidemiologically linked to the disease. A study of the lipopolysaccharides produced by clinical isolates using chemical, 2D ¹H and ¹³C NMR, and MS methods revealed that the O-polysaccharide produced by Cronobacter muytjensii strain 3270, isolated from powdered infant formula from Denmark, was a linear unbranched polymer of a repeating pentasaccharide unit composed of 2-acetamido-2-deoxy-D-galactose (D-GalNAc), 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), 3-acetamido- 3-deoxy-D-quinovose (D-Qui3NAc), L-rhamnose (L-Rha), and D-glucuronic acid (D-GlcA) in equimolar ratio, and has the structure →3)-α-D-GalpNAc-(1→4)-α-D-Quip3NAc-(1→3)-α-L-Rhap-(1→6)-α-D-GlcpNAc-(1→4)-β-D-GlcpA-(1→ The specific structural characteristics of the O-polysaccharides of C. muytjensii may be of value in the identification and tracking of the bacterial pathogen.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences
Peer reviewedYes
NRC numberMACLEAN2009A
NPARC number15336763
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Record identifier86457982-2f63-4a83-9232-a1bf53c119e2
Record created2010-05-21
Record modified2016-05-09
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