Design and fabrication of 3D porous scaffolds to facilitate cell-based gene therapy

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DOIResolve DOI: http://doi.org/10.1089/ten.tea.2006.0418
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TypeArticle
Journal titleTissue Engineering
Volume14
Issue6
Pages10371048; # of pages: 12
Subject3D scaffold; solid free-form fabrication; porogen leaching technique; gene therapy; erythropoietin; mesenchymal stromal cells
AbstractBiomaterials capable of efficient gene delivery by embedded cells provide a fundamental tool for the treatment of acquired or hereditary diseases. A major obstacle is maintaining adequate nutrient and oxygen diffusion to cells within the biomaterial. In this study, we combined the solid free-form fabrication and porogen leaching techniques to fabricate three-dimensional scaffolds, with bimodal pore size distribution, for cell-based gene delivery. The objective of this study was to design micro-/macroporous scaffolds to improve cell viability and drug delivery. Murine bone marrow-derived mesenchymal stromal cells (MSCs) genetically engineered to secrete erythropoietin (EPO) were seeded onto poly-L-lactide (PLLA) scaffolds with different microporosities. Over a period of 2 weeks in culture, an increase in cell proliferation and metabolic activity was observed with increasing scaffold microporosity. The concentration of EPO detected in supernatants also increased with increasing microporosity level.Our study shows that these constructs can promote cell viability and release of therapeutic proteins, and clearly demonstrates their capacity for a dual role as scaffolds for tissue regeneration and as delivery systems for soluble gene products.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Industrial Materials Institute
Peer reviewedYes
NRC number48944
NPARC number15774997
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Record identifier864be268-391b-4060-a174-26cc36be6bf1
Record created2010-07-06
Record modified2016-05-09
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