A novel branching pattern in the lipopolysaccharide expressed by non-typeable Haemophilus influenzae strain 1232

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DOIResolve DOI: http://doi.org/10.1016/j.carres.2013.03.011
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TypeArticle
Journal titleCarbohydrate Research
ISSN0008-6215
Volume378
Pages114122; # of pages: 9
SubjectD-glycero-D-manno-Heptose; Haemophilus influenzae; Lipopolysaccharides; LosA1; Sialyllacto-N-neotetraose; Capillary electrophoresis; Electrospray ionization; Nuclear magnetic resonance spectroscopy; Mass spectrometry; heptose; lipopolysaccharide; oligosaccharide; sialic acid; tetrasaccharide; article; bacterial strain; capillary electrophoresis; carbohydrate analysis; deacylation; dephosphorylation; electrospray mass spectrometry; Haemophilus influenzae; high performance liquid chromatography; hydrolysis; methylation; nuclear magnetic resonance spectroscopy; priority journal; sequence analysis; sialylation; tandem mass spectrometry; Haemophilus influenzae
AbstractWe report the novel branching pattern in lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strain 1232. The strain expressed the β-D-Glcp-(1→4)-[α-D-Galp-(1→4)-β-D-Galp- (1→7)]-D-α-D-Hepp-(1→6)-β-D-Glcp chain linked to the proximal heptose (HepI) of the conserved triheptosyl inner-core moiety of NTHi LPS: L-α-D-HepIIIp-(1→2)-[PEtn→6]-L-α-D-HepIIp-(1→3)- L-α-DHepIp-( 1→5)-[PPEtn→4]-α-Kdop-(2→6)-lipid A. The structure has been elucidated using NMR spectroscopy, electrospray ionization mass spectrometry (ESI-MS) and capillary electrophoresis coupled to electrospray ionization tandem mass spectrometry (CE-ESI-MSn) on O-deacylated LPS and core oligosaccharide (OS) materials, as well as HPLC-ESI-MSn on permethylated, dephosphorylated OS. It was also found that a tetrasaccharide unit bearing sialic acid [α-Neu5Ac-(2→3)-β-D-Galp-(1→4)- β-D-GlcNAcp-(1→3)-β-D-Galp-(1→] could substitute O-4 of the β-D-Glcp linked to HepI. In addition, the distal heptose (HepIII) was substituted by PCho→6-β-D-Galp-(1→ at the O-2 position. © 2013 Published by Elsevier Ltd.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269713
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Record identifier865e9dc6-3703-427e-b742-a099cd4a40e6
Record created2013-12-13
Record modified2016-05-09
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