Cellular polysaccharides of Serratia marcescens

  1. Get@NRC: Cellular polysaccharides of Serratia marcescens (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1139/o67-056
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Journal titleCanadian Journal of Biochemistry
Pages477491; # of pages: 15
AbstractDecapsulated cells of Serratia marcescens were fractionated to yield crude cytoplasmic (I) lipopolysaccharide (II) and cell-wall (III) polysaccharides. Further separation of I yielded dialyzable polysaccharides composed of D-glucose and D-mannose and nondialyzable polysaccharides containing various proportions of D-glucose, D-mannose, L-rhamnose, glucuronic acid, and glucosamine. Fractionation of II by precipitation with Cetavlon–boric acid and by highspeed centrifugation yielded polysaccharides containing D-glucose, L-rhamnose, D-mannose, D-glycero-D-manno-heptose, L-glycero-D-mannose-heptose, glucuronic acid, mannuronic acid, and glucosamine. Analytical data on the various fractions indicated that these polysaccharides included an acidic glucomannan, a rhamnoglucan, and a heptoglucan. Polysaccharides of similar composition have been found in S. marcescens capsular and extracellular polysaccharides in an earlier investigation. Removal of the mucopeptide material from the cell-wall III left an insoluble polysaccharide residue composed of glucose and glucosamine units.
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AffiliationNational Research Council Canada
Peer reviewedYes
NRC number9365
NPARC number21274536
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Record identifier877c80a3-84fd-4016-9139-866cf34a211d
Record created2015-03-16
Record modified2016-05-09
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