Synthesis and characterization of CN-modified protein analogues as potential vibrational contrast agents

Download
  1. Get@NRC: Synthesis and characterization of CN-modified protein analogues as potential vibrational contrast agents (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.bioorg.2006.12.004
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBioorganic Chemistry
ISSN0045-2068
Volume35
Issue3
Pages28493; # of pages: 192
SubjectAntigen-Antibody Reactions; Benzoic Acids; Molecular Structure; Protein Structure, Tertiary; Sensitivity and Specificity; Serum Albumin, Bovine; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrum Analysis, Raman; Staphylococcal Protein A; Staphylococcus aureus; Structure-Activity Relationship; Succinimides; Surface Plasmon Resonance; Vibration
AbstractA recombinant VH single-domain antibody recognizing staphylococcal protein A was functionalized on reactive lysine residues with N-hydroxysuccimidyl-activated 4-cyanobenzoate. Surface plasmon resonance analysis of antibody-antigen binding revealed that modified and unmodified antibodies bound protein A with similar affinities. Raman imaging of the modified antibodies indicated that the benzonitrile group provides vibrational contrast enhancement in a region of the electromagnetic spectrum that is transparent to cellular materials. Thus, the modified single-domain antibody may be amenable to detecting protein A from samples of the human pathogen Staphylococcus aureus using vibronic detection schemes such as Raman and coherent anti-Stokes Raman scattering. The generality of this labeling strategy should make it applicable to modifying an array of proteins with varied structure and function.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Microstructural Sciences; NRC Steacie Institute for Molecular Sciences; NRC Institute for Biological Sciences
Peer reviewedYes
Identifier10056087
NPARC number12327431
Export citationExport as RIS
Report a correctionReport a correction
Record identifier87e5f5e7-ef0c-4969-a865-c6bbb6b2c6fc
Record created2009-09-10
Record modified2017-04-19
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)