A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions

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DOIResolve DOI: http://doi.org/10.1117/12.840500
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Proceedings titleProceedings of SPIE
ConferencePhotonics North 2009, 24 May 2009, Quebec, Canada
Subjectmulti-photon microscopy, atherosclerosis, two photon excited fluorescence, second harmonic generation, intrinsic fluorescence
AbstractIn this study we compare the single-photon autofluorescence and multi-photon emission spectra obtained from the luminal surface of healthy segments of artery with segments where there are early atherosclerotic lesions. Arterial tissue was harvested from atherosclerosis-prone WHHL-MI rabbits (Watanabe heritable hyperlipidemic rabbit-myocardial infarction), an animal model which mimics spontaneous myocardial infarction in humans. Single photon fluorescence emission spectra of samples were acquired using a simple spectrofluorometer set-up with 400 nm excitation. Samples were also investigated using a home built multi-photon microscope based on a Ti:sapphire femto-second oscillator. The excitation wavelength was set at 800 nm with a ~100 femto-second pulse width. Epi-multi-photon spectroscopic signals were collected through a fibre-optics coupled spectrometer. While the single-photon fluorescence spectra of atherosclerotic lesions show minimal spectroscopic difference from those of healthy arterial tissue, the multi-photon spectra collected from atherosclerotic lesions show marked changes in the relative intensity of two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) signals when compared with those from healthy arterial tissue. The observed sharp increase of the relative SHG signal intensity in a plaque is in agreement with the known pathology of early lesions which have increased collagen content.
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AffiliationNRC Institute for Biodiagnostics; NRC Steacie Institute for Molecular Sciences; National Research Council Canada
Peer reviewedYes
NPARC number19697995
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Record identifier8814f441-1bb0-42cf-a252-3aee48a3151d
Record created2012-03-22
Record modified2016-05-09
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