Using gas modifiers to significantly improve sensitivity and selectivity in a cylindrical FAIMS device

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DOIResolve DOI: http://doi.org/10.1007/s13361-014-0878-z
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TypeArticle
Journal titleJournal of the American Society for Mass Spectrometry
ISSN1044-0305
Volume25
Issue7
Pages12741284; # of pages: 11
SubjectDMS; Electrospray; FAIMS; Gas additives; Gas-phase separation
Abstract(Graph Presented) Recent reports describing enhanced performance when using gas additives in a DMS device (planar electrodes) have indicated that comparable benefits are not attainable using FAIMS (cylindrical electrodes), owing to the non-homogeneous electric fields within the analyzer region. In this study, a FAIMS system (having cylindrical electrodes) was modified to allow for controlled delivery of gas additives. An experiment was carried out that illustrates the important distinction between gas modifiers present as unregulated contaminants and modifiers added in a controlled manner. The effect of contamination was simulated by adjusting the ESI needle position to promote incomplete desolvation, thereby permitting ESI solvent vapor into the FAIMS analyzer region, causing signal instability and irreproducible CV values. However, by actively controlling the delivery of the gas modifier, reproducible CV spectra were obtained. The effects of adding different gas modifiers were examined using 15 positive ions having mass-to-charge (m/z) values between 90 and 734. Significant improvements in peak capacity and increases in ion transmission were readily attained by adding acetonitrile vapor, even at trace levels (≤0.1%). Increases in signal intensity were greatest for the low m/z ions; for the six lowest molecular weight species, signal intensities increased by ∼10- to over 100-fold compared with using nitrogen without gas additives, resulting in equivalent or better signal intensities compared with ESI without FAIMS. These results confirm that analytical benefits derived from the addition of gas modifiers reported with a uniform electric field (DMS) also are observed using a non-homogenous electric field (FAIMS) in the analyser region.
Publication date
PublisherSpringer International Publishing
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedYes
NRC numberNRC-ACRD-55852
NPARC number21272653
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Record identifier88804470-0c8b-46ef-921c-8b66c5d47a82
Record created2014-12-03
Record modified2016-05-09
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